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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)

To view a copy of this licence, visit About this article Cite this article Schmieder, H., Leischner, C., Piotrowsky, A

Am J Physiol 1997;273(5 Pt 2):R1771-R1778

Function and therapeutic value of astrocytes in neurological diseases

By comparing the migration patterns of bound and free DNA, researchers can determine the binding affinity and specificity of the protein for the DNA sequence

Transcriptional activation of the proglucagon gene by lithium and -catenin in intestinal endocrine L cells